chercheur en entreprise, R&D du secteur privé*

Coordonnées

Techniques maîtrisées

Bacterial cultures: Handling and transformation Recombination DNA techniques: DNA cloning and expression Protein purification (FPLC) RFLP-PCR, Spoligo typing, PCR mutagenesis technique Enzymatic and Inhibition assays Hemagglutination Inhibition (HI) and Micro-neutralization (MN) assays Crystallogenesis Other common techniques in molecular biology laboratory

Compétences

- Social skills: Teamwork, Intercultural skill - Computer skills and competences: + Good command of Microsoft OfficeTM tools (WordTM, ExcelTM and PowerPointTM + Basic knowledge of graphic design applications (PhotoShopTM), Bioinformatics tools

Doctorat

Intitulé : Biologie-Santé - Spécialité Maladies Infectieuses
1ère inscription en thèse : Septembre 2015
École doctorale : Sciences de la Vie et de la Santé
Date de soutenance de la thèse : 22 Octobre 2020
Sujet : Etude des enzymes de réplication de coronavirus: assemblage, mécanisme et inhibition
Directeur de thèse : Bruno CANARD
Co-directeur :
Unité de recherche : AFMB - Architecture et Fonction des Macromolécules Biologiques
Intitulé de l'équipe : AFMB - Réplicases Virales : Structure Mécanisme et Drug-Design

Master

Intitulé : Master of Science
Septembre 2015 - University of Pavia

Langues vivantes

Anglais : C1 - Avancé
Italien : A2 - Élémentaire
Français : A2 - Élémentaire
Vietnamien : Maternel

Production scientifique

  • Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.
    PNAS 2018
    Ferron F, Subissi L, Silveira De Morais AT, Le NTT , Sevajol M, Gluais L, Decroly E, Vonrhein C, Bricogne G, Canard B, Imbert I
    https://www.ncbi.nlm.nih.gov/pubmed?term=29279395
  • Low fidelity of SARS Coronavirus polymerase complex is restored by the nsp14 Exonuclease
    International Conference on Antiviral Research - ICAR 2018 2018
    Nhung Le, Lorenzo Subissi, Ana Theresa Silveira De Morais, Francois Ferron, Isabelle Imbert, Bruno Canard
  • Processivity and Fidelity of SARS Coronavirus (SARS-CoV) polymerase complex
    26th Annual Meeting of Doctoral School 2018
    Nhung Le, Lorenzo Subissi, Ana Theresa Silveira De Morais, Francois Ferron, Isabelle Imbert, Bruno Canard
  • Remdesivir and SARS-CoV-2: structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
    ASV 2020
    Shannon A., Nhung Le, Selisko B., Eydoux C, Alvarez K., Guillemot JC, Decroly E., Peersen O., Ferron F., and Canard B
    https://www.sciencedirect.com/science/article/pii/S0166354220302072
  • Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis
    Nature Communication 2020
    A. Shannon, B. Selisko, NTT Le, J. Huchting, F. Touret, G. Piorkowski, V. Fattorini, F. Ferron, E. Decroly, C Meier, B. Coutard, O. Peersen & B. Canard
  • A Fluorescence-based High Throughput-Screening assay for the SARS-CoV RNA synthesis complex
    preprint 2020
    Eydoux C., Fattorini V., Shannon A., Thi-Tuyet-Nhung Le, Didier B, Canard B., Guillemot JC
  • Signatures and mechanisms of efficacious therapeutic ribonucleotides against SARS-CoV-2 revealed by analysis of its replicase using magnetic tweezers
    Nature 2020
    Mona Seifert1, Subhas Chandra Bera, Pauline van Nies, Robert N. Kirchdoerfer , Ashleigh Shannon, Thi-Tuyet-Nhung Le, Tyler L. Grove, Flávia S. Papini , Jamie J. Arnold, Steven C. Almo , Bruno Canard, Martin Depken,, Craig E. Cameron, and David Dulin
  • Fast and inaccurate selection of nucleotides and Favipiravir analogues at the SARS- CoV RNA polymerase active site
    in preparation 2020
    Nhung Thi-Tuyet Le, Johanna Huchting, Lorenzo Subissi, Barbara Selisko, Ashleigh Shannon, Cecilia Eydoux, Karine Alvarez, Isabelle Imbert, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Chris Meier, Francois Ferron, and Bruno Canard