chercheur en entreprise, R&D du secteur privé*
Coordonnées
Techniques maîtrisées
Bacterial cultures: Handling and transformation
Recombination DNA techniques: DNA cloning and expression
Protein purification (FPLC)
RFLP-PCR, Spoligo typing, PCR mutagenesis technique
Enzymatic and Inhibition assays
Hemagglutination Inhibition (HI) and Micro-neutralization (MN) assays
Crystallogenesis
Other common techniques in molecular biology laboratory
Compétences
- Social skills: Teamwork, Intercultural skill
- Computer skills and competences:
+ Good command of Microsoft OfficeTM tools (WordTM, ExcelTM and PowerPointTM
+ Basic knowledge of graphic design applications (PhotoShopTM), Bioinformatics tools
Doctorat
Intitulé : Biologie-Santé - Spécialité Maladies Infectieuses
1ère inscription en thèse :
Septembre 2015
École doctorale :
Sciences de la Vie et de la Santé
Date de soutenance de la thèse :
22 Octobre 2020
Sujet :
Etude des enzymes de réplication de coronavirus: assemblage, mécanisme et inhibition
Directeur de thèse :
Bruno CANARD
Co-directeur :
Unité de recherche :
AFMB - Architecture et Fonction des Macromolécules Biologiques
Intitulé de l'équipe :
AFMB - Réplicases Virales : Structure Mécanisme et Drug-Design
Master
Intitulé : Master of Science
Septembre 2015 - University of Pavia
Langues vivantes
Anglais : C1 - Avancé
Italien : A2 - Élémentaire
Français : A2 - Élémentaire
Vietnamien : Maternel
Production scientifique
-
Structural and molecular basis of mismatch correction and ribavirin excision from coronavirus RNA.
PNAS
2018
Ferron F, Subissi L, Silveira De Morais AT, Le NTT , Sevajol M, Gluais L, Decroly E, Vonrhein C, Bricogne G, Canard B, Imbert I
https://www.ncbi.nlm.nih.gov/pubmed?term=29279395
-
Low fidelity of SARS Coronavirus polymerase complex is restored by
the nsp14 Exonuclease
International Conference on Antiviral Research - ICAR 2018
2018
Nhung Le, Lorenzo Subissi, Ana Theresa Silveira De Morais, Francois Ferron, Isabelle Imbert, Bruno Canard
-
Processivity and Fidelity of SARS Coronavirus (SARS-CoV)
polymerase complex
26th Annual Meeting of Doctoral School
2018
Nhung Le, Lorenzo Subissi, Ana Theresa Silveira De Morais, Francois Ferron, Isabelle Imbert, Bruno Canard
-
Remdesivir and SARS-CoV-2: structural requirements at both nsp12 RdRp and nsp14 Exonuclease active-sites
ASV
2020
Shannon A., Nhung Le, Selisko B., Eydoux C, Alvarez K., Guillemot JC, Decroly E., Peersen O., Ferron F., and Canard B
https://www.sciencedirect.com/science/article/pii/S0166354220302072
-
Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis
Nature Communication
2020
A. Shannon, B. Selisko, NTT Le, J. Huchting, F. Touret, G. Piorkowski, V. Fattorini, F. Ferron, E. Decroly, C Meier, B. Coutard, O. Peersen & B. Canard
-
A Fluorescence-based High Throughput-Screening assay for the SARS-CoV RNA synthesis complex
preprint
2020
Eydoux C., Fattorini V., Shannon A., Thi-Tuyet-Nhung Le, Didier B, Canard B., Guillemot JC
-
Signatures and mechanisms of efficacious therapeutic ribonucleotides against SARS-CoV-2 revealed by analysis of its replicase using magnetic tweezers
Nature
2020
Mona Seifert1, Subhas Chandra Bera, Pauline van Nies, Robert N. Kirchdoerfer , Ashleigh Shannon, Thi-Tuyet-Nhung Le, Tyler L. Grove, Flávia S. Papini , Jamie J. Arnold, Steven C. Almo , Bruno Canard, Martin Depken,, Craig E. Cameron, and David Dulin
-
Fast and inaccurate selection of nucleotides and Favipiravir analogues at the SARS- CoV RNA polymerase active site
in preparation
2020
Nhung Thi-Tuyet Le, Johanna Huchting, Lorenzo Subissi, Barbara Selisko, Ashleigh Shannon, Cecilia Eydoux, Karine Alvarez, Isabelle Imbert, Jean-Claude Guillemot, Etienne Decroly, Olve Peersen, Chris Meier, Francois Ferron, and Bruno Canard