Madame Sophie PERRIN / Docteur

Compétences

Multidisciplinary exchanges with clinicians, pharmacologists, virologists and statiticians / Clinical trial standard requirements Supervision of research assistant Management of the dedicated budget line Effective reporting experiments (e.g eLNB software, meeting presentations) Effective team member

Techniques maîtrisées

Molecular techniques: cDNA cloning, directed mutagenesis, PCR, Southern Blotting Cell cuture including peripheral blood mononuclear cells (PBMC), fibroblasts, HEK293T (human embryonic kidney), HEK MRSII (HEK293 cell allowing a better adhesion to dishes), SW480 or 620 (colon cancer cell line). Cell assays (e.g. Cell viability through propidium iodure, trypan bleu, cell transfection, raft purification, drug assay toxicity on PBMC and fibroblasts, mitochondrial function and morphological assay). Protein analysis: SDS PAGE gels, western blotting, immunocytochemistry, co-immunoprecipitation Microscopy techniques: Fluorescence microscopy, confocal microscopy Flow cytometry analysis (e.g. cell viability (Annexin V/ 7AAD), PBMC phenotyping, mitochondrial function assay ( CM-H2-DCFDA, JC-1), lamins detection) using FC500 or Navios flow cytometer and FlowJo software analysis.