Madame Sophie PERRIN / PhD

Recherche appliquée / Industrie Pharmaceutique

Contact details

Professional experience

Contract type : Entreprises

Organization or company : Laboratoire Prenyl B

Website : http://www.prenylbio.com/fr/2011-07-18-15-48-25.html

City : Marseille

Country : FRANCE

Jobs

Availability : 3 2013

Additional information : Travelling, Photos

Mobility : Europe

Research : OUI

Skills

Multidisciplinary exchanges with clinicians, pharmacologists, virologists and statiticians / Clinical trial standard requirements Supervision of research assistant Management of the dedicated budget line Effective reporting experiments (e.g eLNB software, meeting presentations) Effective team member

Techniques

Molecular techniques: cDNA cloning, directed mutagenesis, PCR, Southern Blotting Cell cuture including peripheral blood mononuclear cells (PBMC), fibroblasts, HEK293T (human embryonic kidney), HEK MRSII (HEK293 cell allowing a better adhesion to dishes), SW480 or 620 (colon cancer cell line). Cell assays (e.g. Cell viability through propidium iodure, trypan bleu, cell transfection, raft purification, drug assay toxicity on PBMC and fibroblasts, mitochondrial function and morphological assay). Protein analysis: SDS PAGE gels, western blotting, immunocytochemistry, co-immunoprecipitation Microscopy techniques: Fluorescence microscopy, confocal microscopy Flow cytometry analysis (e.g. cell viability (Annexin V/ 7AAD), PBMC phenotyping, mitochondrial function assay ( CM-H2-DCFDA, JC-1), lamins detection) using FC500 or Navios flow cytometer and FlowJo software analysis.

PhD

Title : Pathologie Humaine - Spécialité Génétique humaine

First registration date : 7 November 2008 / 4A

Doctoral school : Sciences de la Vie et de la Santé

Thesis defense date : 14 December 2012

Subject : Vieillissement, infection par le VIH-1 & traitements antirétroviraux

Thesis supervisor : CAU Pierre

Thesis co-supervisor : ROLL Patrice

Research unit : GMGF - Génétique Médicale et Génomique Fonctionnelle

Team : Génétique des maladies neuromusculaires et des laminopathies

Master's degree

Title : Ingénieur Biotechnologie

October 2008 - ESIL

Publications

  • HIV-1 Infection and First Line ART Induced Differential Responses in Mitochondria from Blood Lymphocytes and Monocytes: The ANRS EP45 "Aging" Study
    PLoS One, 2012
    Sophie Perrin, Jonathan Cremer, Patrice Roll, Olivia Faucher, Amélie Ménard, Jacques Reynes, Pierre Dellamonica, Alissa Naqvi, Joëlle Micallef, Elisabeth Jouve, Catherine Tamalet, Caroline Solas, Christel Pissier, Isabelle Arnoux, Corine Nicolino-Brunet, Léon Espinosa, Nicolas Lévy, Elise Kaspi, Andrée Robaglia-Schlupp, Isabelle Poizot-Martin, Pierre Cau.
    http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041129
  • HIV protease inhibitors do not cause the accumulation of prelamin A in PBMCs from patients receiving first line therapy: the ANRS EP45 “Aging” study
    PLoS One, 2012
    Sophie Perrin, Jonathan Cremer, Olivia Faucher, Jacques Reynes, Pierre Dellamonica, Joëlle Micallef, Caroline Solas, Bruno Lacarelle, Charlotte Stretti, Elise Kaspi, Andrée Robaglia-Schlupp, Corine Nicolino-Brunet Catherine Tamalet, Nicolas Lévy, Isabelle Poizot-Martin, Pierre Cau, Patrice Roll.
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Languages

Anglais : C2 - Courant

Italien : B2 - Intermédiaire supérieur