Madame Sarah PYRTEK / Doctorant

Compétences

Participation a Euroglia et FAAST, poster and oral presentations Encadrement d'une staggiere M2 pour 6 mois

Techniques maîtrisées

Western blotting and ELISA for protein detection and quantification. Immunohistochemistry (IHC) and immunocytochemistry (ICC) for spatial visualization of protein expression in cells and tissues. RNA scope for high-resolution detection of gene expression at the single-cell level. PCR and RT-qPCR for DNA and RNA analysis to confirm gene editing and measure transcript expression. Calcium live imaging to assess cellular activity and intracellular calcium dynamics in differentiated astrocytes and/or neruons. Fiji image analysis, quPath image analysis RNA/DNA extraction Plasmid amplification Confocal microscopy, apotome, Axio Scan, Life imaging iPSC culture: - maintenance - amplifcation - freezinf/thawing - gene editing - differentiation into neuronal lines (astrocytes, neurons, NPCs) Gene diting in iPSC: - guide RNA design - designing CRIPSR KO, CRIPSR KI, CRIPR a, CRIPSR i, shRNA - virus design - virus transduction - elctroporation - clonal isolation - RNA sequencing