Madame Sarah PYRTEK / PhD student

Skills

Participation a Euroglia et FAAST, poster and oral presentations Encadrement d'une staggiere M2 pour 6 mois

Techniques

Western blotting and ELISA for protein detection and quantification. Immunohistochemistry (IHC) and immunocytochemistry (ICC) for spatial visualization of protein expression in cells and tissues. RNA scope for high-resolution detection of gene expression at the single-cell level. PCR and RT-qPCR for DNA and RNA analysis to confirm gene editing and measure transcript expression. Calcium live imaging to assess cellular activity and intracellular calcium dynamics in differentiated astrocytes and/or neruons. Fiji image analysis, quPath image analysis RNA/DNA extraction Plasmid amplification Confocal microscopy, apotome, Axio Scan, Life imaging iPSC culture: - maintenance - amplifcation - freezinf/thawing - gene editing - differentiation into neuronal lines (astrocytes, neurons, NPCs) Gene diting in iPSC: - guide RNA design - designing CRIPSR KO, CRIPSR KI, CRIPR a, CRIPSR i, shRNA - virus design - virus transduction - elctroporation - clonal isolation - RNA sequencing