Tau protein aggregation and α-synuclein dysfunction: development of new in vitro and in vivo models to study neurodegenerative diseases

Coordonnées

Techniques maîtrisées

­ Cellular biology: •Cell culture: SHSY, primary cultures of mouse hippocampal neurons. Transfection and nucleofection. •Microbiology: liquid and solid culture of bacterias, isolation and transformation. •Microscopy: fluorescence and light microscopy. ­ Molecular biology: •Vector design: primers design, digestions, ligations, cloning. •Cloning: transformation, RNA extraction, reverse transcription, PCR, •DNA purification, DNA gel analysis, quantification and extraction. ­ Biochemistry: •Western blot, (co­)immunoprecipitation. ­ Animal manipulation: •Handling, feeding, breeding, marking and genotyping of transgenics; dissection of embryos for primary cultures of neurons. •Differential staining of tissue sections. Imunnohistochemistry. •rodent gait analysis by Catwalk system ­ Analytical methods: •Titrations, spectrophotometry, chromatography (ion exchange, exlusion, FPLC, HPLC), isothermal titration calorimetry, differential scanning calorimetry, differential scanning fluorimetry, analytical ultracentrifugation, turbidimetry, dynamic light scattering.

Doctorat

Intitulé : Biologie-Santé - Spécialité Oncologie
1ère inscription en thèse : October 2014
École doctorale : Sciences de la Vie et de la Santé
Date de soutenance de la thèse : 6 July 2018
Sujet : synucléines et protéine tau dans les maladies neurodégénératives
Directeur de thèse : Vincent PEYROT
Co-directeur : Nataliya NINKINA
Unité de recherche : INP - Institut de Neurophysiopathologie
Intitulé de l'équipe : Team 2 : Redox Microenvironment, Cytoskeleton and colorectal tumor progression

Master

Intitulé : Biotechnology
July 2014 - East Siberia State University of Technology and Management

Langues vivantes

Production scientifique

  • Sequential binding of calcium ions to B-repeat domain of SdrD from Staphylococcus aureus.
    Canadian Journal of Microbiology 2015
    Andrei Yu. Roman, François Devred, Vladimir M. Lobatchov, Alexander A. Makarov, Vincent Peyrot, Aslan A. Kubatiev, Philipp O. Tsvetkov
    http://www.ncbi.nlm.nih.gov/pubmed/26639248
  • Generation of mouse lines with conditionally or constitutively inactivated Snca gene and Rosa26-stop-lacZ reporter located in cis on the mouse chromosome 6
    Transgenic Research 2016
    Andrei Yu. Roman, Galina Limorenko, Alexey A. Ustyugov, Tatiana V. Tarasova, Ekaterina A. Lysikova, Vladimir L. Buchman, Natalia Ninkina
    https://www.ncbi.nlm.nih.gov/pubmed/27838898#
  • The role of alpha-synuclein in the development of the dopaminergic neurons in the substantia nigra and ventral tegmental area
    Doklady Biological Sciences 2016
    T. V. Tarasova, O. A. Lytkina, A. Yu. Roman, S. O. Bachurin, A. A. Ustyugov
    https://www.ncbi.nlm.nih.gov/pubmed/27021360
  • Zinc binding to RNA recognition motif of TDP-43 induces the formation of amyloid-like aggregates
    Scientific Reports 2017
    Garnier C, Devred F, Byrne D, Puppo R, Roman AY, Malesinski S, Golovin AV, Lebrun R, Ninkina NN, Tsvetkov PO.
    https://www.ncbi.nlm.nih.gov/pubmed/28754988#
  • Gamma-carboline inhibits neurodegenerative processes in a transgenic model of amyotrophic lateral sclerosis
    Doklady Biochemistry and Biophysics 2015
    E. V. Bronovitsky, A. V. Deikin, T. G. Ermolkevich, A. B. Elyakov, E. N. Fedorov, E. R. Sadchikova, I. L. Goldman, R. K. Ovchinnikov, A. Yu. ROMAN, I. V. Khritankova, M. S. Kukharsky, V. L. Buchman, S. O. Bachurin, A. A. Ustyugov
    https://link.springer.com/article/10.1134%2FS1607672915030138
  • The FUS protein: Physiological functions and a role in amyotrophic lateral sclerosis
    Molecular Biology 2017
    Efimova AD, Ovchinnikov RK, ROMAN AY, Maltsev AV, Grigoriev VV, Kovrazhkina EA, Skvortsova VI.
    https://www.ncbi.nlm.nih.gov/pubmed/28707655
  • Differential scanning calorimetry of plasma in glioblastoma: toward a new prognostic / monitoring tool
    Oncotarget 2018
    Philipp O Tsvetkov, Emeline Tabouret, ANDREI Yu ROMAN, Sylvie Romain, Céline Bequet2, Olga Ishimbaeva, Stéphane Honoré, Dominique Figarella-Branger, Olivier Chinot, François Devred
    https://www.ncbi.nlm.nih.gov/pubmed/29507697
  • Detection of autoantibodies to potentially amyloidogenic protein, gamma-synuclein, in the serum of patients with amyotrophic lateral sclerosis and cerebral circulatory disorders
    Doklady Biochemistry and Biophysics 2017
    A.Yu. ROMAN, E.A. Kovrazhkina, O.D. Razinskaya, M.S. Kukharsky, A.V. Maltsev, R.K. Ovchinnikov, O.A. Lytkina, A.P. Smirnov, A.A. Moskovtsev, Y.V. Borodina, A.P. Surguchov, A.A. Ustyugov, N.N. Ninkina, V.I. Skvortsova
    https://link.springer.com/article/10.1134%2FS1607672917010197
  • Temperature-dependent reversible oligomerization of tau
    EuroTau, Lille, France 2018
    A.Y. ROMAN, F. Devred, D. Byrne, N. Ninkina, V. Peyrot, P.O. Tsvetkov.