Monsieur Andrei ROMAN / Docteur

Techniques maîtrisées

­ Cellular biology: •Cell culture: SHSY, primary cultures of mouse hippocampal neurons. Transfection and nucleofection. •Microbiology: liquid and solid culture of bacterias, isolation and transformation. •Microscopy: fluorescence and light microscopy. ­ Molecular biology: •Vector design: primers design, digestions, ligations, cloning. •Cloning: transformation, RNA extraction, reverse transcription, PCR, •DNA purification, DNA gel analysis, quantification and extraction. ­ Biochemistry: •Western blot, (co­)immunoprecipitation. ­ Animal manipulation: •Handling, feeding, breeding, marking and genotyping of transgenics; dissection of embryos for primary cultures of neurons. •Differential staining of tissue sections. Imunnohistochemistry. •rodent gait analysis by Catwalk system ­ Analytical methods: •Titrations, spectrophotometry, chromatography (ion exchange, exlusion, FPLC, HPLC), isothermal titration calorimetry, differential scanning calorimetry, differential scanning fluorimetry, analytical ultracentrifugation, turbidimetry, dynamic light scattering.