Madame Stéphanie CORNEN / Docteur

Techniques maîtrisées

Molecular biology : • Primer Design for PCR • RT-PCR, RTQ-PCR • Sequencing • Cloning (ligation; In-Fusion Cloning, Ozyme • Mutagenesis • DNA microarray (CGH Microarrays and DNA methylation Microarrays, Agilent technologies Cellular Biology • Cell culture • Transfection • Flow cytometry Biochemistry • Protein production in prokaryotes and eukaryotes • Protein purification (HPLC) • Protein assay • Western Blot Biophysics • Transmission electron microscopy • Co-sedimentation assay • Isothermal titration calorimetry (Initiation at the Engelhardt Institute, Russian Academy of Sciences, Moscow)