Monsieur Babu Roshan PADMANABHAN / Docteur


Major Projects: 1. PhD Thesis : Taxono-genomics, a strategy incorporating genomic data into the taxonomic description of human bacteria Supervisor : Prof. Pierre-Edouard Fournier Project Tenure : Nov 2011 – will finish by Nov 2014 Place : Aix-Marseille University, URMITE, Faculty de Medicine, Marseille, France Accomplished : more than 10 publications including two review, learned Next generation sequencing, de novo assembly, developed a pipeline which the lab uses. 2. Project Title: Transgenic Overexpression of mouse expressing 12R-Lipoxygenase and Phylogenetic Studies of Lipoxygenase gene family. Supervisor : Prof. P Reddanna Project Tenure : May 2006 – Oct 2010 Place : Hyderabad Central University, Animal Science Department, Hyderabad, India Accomplished : poster presentation accepted in European Conference on Computational Biology at Ghent , Belgium - September 26-29, 2010, Learned the techniques of cell culture and embryonic stem cell culture. 3. Project Title: Sequencing, Annotation and Phylogenetic studies of full-length molecular clone of HIV-1 Subtype C. Supervisor : Dr. Udaykumar Ranga Project Tenure : Oct 2004 – Feb 2006 Place : Jawaharlal Nehru Centre for Advanced Scientific Research, Molecular Virology Laboratory, Molecular Biology and Genetics Unit. Bangalore, India Accomplished : co authored an article in journal Retrovirology, done several HIV viral assembly and annotation , developed lab website. 4. Thesis Title : Identification of dispersed repeat families in genome. Course : Advanced Post Graduate Diploma in Bioinformatics. Supervisor : Prof Alok Bhattacharya Project Tenure : Jan 2004 – April 2004. Place : Bioinformatics Center, JNU, New Delhi, India. Accomplished : learned programming in perl, bioperl, unix shell scripting. Developed a script to identify and classify the dispersed repeat elements. 5. Thesis Title : Screening of marine algae for Hemagglutinins and the detection of Hemagglutination titre. Course : M.Sc in Marine Biotechnology. Supervisor : Dr. Urmila Barros. Project Tenure : July 2001 – April 2002. Place : Department of Marine Sciences and Biotechnology, Goa University, Goa, India. Minor Projects (In Bioinformatics) 1. Microarray analysis of data and clustering by using K-mean technique and visualization by Principle Component Analysis (PCA). 2. Find out coding and non-coding region using 1st order Markov model. 3. Making a relational database using Oracle8i. 4. Identification of gene families in microbial genomes.

Adresse professionnelle

URMITE - UMR CNS 6236, IRD 198 Université de la Méditerranée Unités Rickettsies - Centre de Référence Faculté de Médecine 27, Boulevard Jean Moulin, 13005 Marseille , FRANCE

+337811289352

Expérience professionnelle

Type de contrat : Contrat post-doctoral en organisme de recherche (EPST, EPIC, EPA)

Organisme de rattachement ou entreprise : Institut des Maladies Métaboliques et Cardiovasculaires.

Ville : Toulouse

Pays : FRANCE

Emplois

Mobilité ? : Anywhere in Europe

Disponibilité : 12 2014

Informations complémentaires : Comparative Genomic and Proteomic Analysis Computational PhyloGenomics Viral Genome Evolution Stem Cell Biology and Transcription regulation

Mobilité géographique : Europe

Recherche : OUI

Compétences

Bioinformatics , Genomics, Phylogentics, Scientific Programming in Perl and Python

Techniques maîtrisées

Domain Specific Knowledge Analysis of Next Generation Sequence Data [Illumina , SOLiD, 454] for metagenomics as well as whole genome assembly. Hands on experience in Roche/454 Sequencing. de novo assembly tools - Velvet, Newbler, Mira, CLC. Setting up and maintaining local database for Handling NGS Data, Blast database etc. Knowledge of the algorithms behind BLAST, BLAT, OrthoMCL etc. Developing pipelines for Phylogenomics [MAGI : Developed a pipeline for whole genome comparison (manuscript under preparation) ]. Large Scale Genome Analysis using Galaxy browser. Intermediate level Linux Administration. And an in depth knowledge of a large number of tools, resources and databases available over the WWW for biological sequence analysis. Familiar with the following Bioinformatics tools BLAST [standalone, web] BLAT DNASTAR EMBOSS CLUSTAL-W Phylogentic softwares like Phylip,PhyML,MEGA DAMBE Oligo6 for Primer designing Phred, Phrap, Consed HMMER RasMol,MSI(Insight) ADT,VMD,NAMD Repeat Finder Swiss PDB Viewer Large Scale Genome Analysis by Galaxy browser And an in depth knowledge of a large number of tools, resources and databases available over the WWW for biological analysis. Statistical Softwares : MINITAB, Octave,R Molecular Biology tools and techniques Genomic DNA, RNA isolation Plasmid DNA isolation Molecular Cloning / Recombineering. PCR (Hot start, Nested PCR, RT-PCR) cDNA Library construction. mouse ES cell /Animal Cell culture MALDI-TOF, SDS PAGE, ELISA, Western /Southern Blotting Protein purification techniques Reporter gene assay [SEAP Assay, -gal assay]. DNA Sequencing [ABI, Roche/454]. Tissue Culture techniques Growth and maintenance of cell lines (HaCaT,CHO,BSF, HEK293) Culturing of mouse Embryonic Stem Cells (R19) Fibroblast cell line (BSF and HaCat cell lines) Electroporation of knockout vectors Making Embryoid Bodies by Hanging drop

Doctorat

Titre : Biologie-Santé - Spécialité Maladies Infectieuses

1ère inscription en thèse : 28 novembre 2011 / 3A

Ecole doctorale : Sciences de la Vie et de la Santé

Date de soutenance de thèse : 8 décembre 2014

Sujet : Taxono-génomique, une stratégie intégrant les données génomiques dans la description taxonomique des bactéries humaines

Directeur de thèse : FOURNIER Pierre-Edouard

Co-directeur :

Unité de recherche : URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales

Equipe : Unité des Rickettsies et des pathogènes émergents

Master

Titre : Goa Univeristy

avril 2002 - Goa Univeristy

Langues

Anglais : B2 - Intermédiaire supérieur

Hindi : B2 - Intermédiaire supérieur

Malayalam : C2 - Maternel