Monsieur Ludovic PETIT / Docteur

Compétences

Electronic design & implementation: 14 channels MEA amplifier for coupled in vitro MEA/Patch-clamp recordings Electronics skills: Basics in analog circuit design, filters, power supply, ADC/DAC and I2C bus, design of systems using 16 bits Microchip PICs, CPLD (in learning),. PCB design, manufacture and testing (2 layers). Reading of technical specifications and drawings. Software: Matlab, ImageJ, Linux(Ubuntu/Debian), MS Office suite, Adobe Photoshop, Icprog, Visual Molecular Dynamics, Quartus II (Altera), Kicad, C language (basis, in learning).

Techniques maîtrisées

Electrophysiology: In vitro microelectrode array recordings on acute brain slices, network events characterization and localization of their initiation sites, patch clamp recordings from acute brain slices, cell cultures, validation of in vitro channels expression (Kir2.1, ChR2) Biology & physiology: Acute brain slices preparation using vibratome, organotypic and neuronal primary cultures preparation, cell cultures maintenance, in utero electroporation Histology: Microtomy, cryotomy, immunohistochemistry techniques and Confocal microscopy on Leica SP5, ex-vivo subcellular localization of fluorescent tagged channels after viral expression Molecular biology: gene cloning and fusion proteins production, western blot