Monsieur Quentin CHARRAS / Docteur

Techniques maîtrisées

Biochimie: Purification de protéines, Electro Mobility Shift Assay (EMSA), western blot, SDS page, Native-PAGE, extraction membrane photosynthétique. Electron microscopy : Resin embedded sample and EM-grid prep, observation under TEM.Immunoelectron microscopy using cryosections. Cryo-EM: Cryo-EM grid preparation. Biophysics : DUAL-PAM chlorophyll fluorescence, 77k fluorescence. Beginner for ECS assays. Bioinformatics: Sequence alignment and analysis, phylogenetic tree building and analysis. Pymols software for 3D protein model visualisation and substitution. Botany: Wide knowledges on plants taxonomy, ecology and identification. Biologie moléculaire: extraction et prufication D'ADN, design de primer PCR, PCR, RTqPCR. Microbiologie: Transformation bactéries compétentes, culture liquide et solide de levures et bactéries. Biologie cellulaire: culture de fibroblastes, cytométrie, microscopie optique. Médicale: échantillonnage matériel biologique. Single particule Cryo-electron-microscopy: sample preparation, data collection and model building.