Monsieur Nourdine CHAKOURI / PhD

Techniques

Physiology: - Rodent handling and in-vivo animal manipulation, tissue excision, pharmacological treatment - Atrial and ventricular cardiomyocytes isolation using Langendorff system (rat and mouse) - Excitation-contraction coupling study using IonOptix system and study of cardiac contractile apparatus property in permeabilized cardiomyocytes, force measurements and activated force/Ca2+ concentration relationship Molecular biology and histology: - Tissue lysis, western blot, immunoprecipitation, RT PCR, biochemical assays - Histology: cryosections, immunohistochemistry, muscle phenotyping Tools and software: Office Pack, ClampFit, AxioVisio, ImageJ, Odyssey, IonWizard, GraphPad Prism, Origin Pro Lab, data analysis and statistic Languages: English (fluent), French (native language)