Madame IRENE SANCHEZ MARTIN / PhD

Techniques

Laboratory Techniques/Skills Molecular biology and biochemistry -DNA and RNA extraction -Real-Time PCR, methods based PCR -Molecular -Cloning -Western-blot and electrophoresis (Agarose and Acrylamide) -Sequencing using 3130XL Genetic Analyzers and Sanger sequencing -Análisis Sequences -Design Primers -Flow cytometry -Fidelity assays of polymerases using labeled nucleotides and antiviral drugs -Expression and purification of proteins, site-directed mutagenesis, transcription in vitro. Serology for microbiology and parasitology. -ELISA-Tests development for different veterinary species. -Immunodiffusion -Immunofluorescence Hematology -Analysis data and development of Advia 120 XT2000i Siemens and Sysmex for hormon analysis. -Analysis of clinical parameters like ALT, GLDH, AP, TP, Alb, Fructosamine, BUN, Create, UPC, TT4, CK, K, PO4 , Fe Cell culture and virology (work in security level P2 and P3) -Cell lines with which I have worked:RK 13, Huh-7.5 cells and Lunet. -Freezing and storage of cells -Cell maintenance -Cells infection and virus titration Virus that I worked -Equine arteritis virus -Aujeszky -Hepatitis C virus Good used of Optical microscope inclusive -Inverted microscope -Fluorescence microscop