Monsieur Sushovan DAM / PhD

Skills

Creativity, Critical thinking, Self-learning

Techniques

1. Microbiology- Isolation and characterization of microbial cultures (fungal and bacterial), Biofilm estimation by crystal violet assay on different surfaces, Handling anaerobic microbes, Serial dilution spotting assay for high throughput characterizations, Bacterial conjugation, General microbiological techniques 2. Biochemistry- SDS-PAGE, Protein purification, Western blotting 3. Microscopy- Scanning electron microscopy, Confocal microscopy 4. Molecular biology- Yeast and bacterial cell transformation, Cloning, Fusion and Colony PCR, qPCR/ RT-PCR 5. Animal cell culture- Maintenance and passaging of cell lines, siRNA transfection, Infection assays, Monoclonization of antibodies 6. Genomics- Annotation and comparison of whole microbial genomes using ARTEMIS tool