Madame Leda LACARIA / PhD

Skills

Programming languages: C, C++, Matlab, LabVIEW Platforms: Linux, Windows Tools: Office, Latex, Visual Studio, QtiPlot, OriginPro, Imagej, NIS Elements Confocal Software, Asylum research AFM Software, Matlab, JPK Data Processing, LabVIEW, Arduino Experiences in organizing scientific seminars: Partecipation in the PhD students committee ivolved in the organization of “Centuri Tutored seminars”: Proposing a speaker for the Centuri tutored seminars, studying two scientific papers sent by each speaker, discussing with other PhD students about the papers before the seminar, attending the seminar, meeting with each speaker after the seminar with the other PhD students to have an open discussion.

Techniques

Use of AFM (Atomic Force Microscope) in air and in fluids Imaging, Force Spectroscopy, Quantitative Imaging and peak-force mode on biological systems: proteins, cells and tissues Type of AFM used: Multimode AFM, Asylum Research MFP-3D AFM , JPK NanoWizard AFM with Ultra Speed updating, Bruker Bioscope Resolve, SS-NEX High-Speed AFM and PS-NEX High Speed AFM coupled with a Spinning Disk (Toshio Ando prototype, Research Institute of Biomolecule Metrology Co.,Ltd.) AFM probe realization using a cantilever and a micrometric glass bead Use of Confocal Microscope and Spinning Disk Image processing and data analysis Cell culture Cell Micropatterning on glass coverslips and on soft PDMS gels for Traction Force Microscopy using PRIMO and UV lamp PRIMO Microscope Traction Force Microscopy Super Resolution Microscopy