Monsieur Mauro VEDELAGO / PhD

Skills

- Self-confidence - Flexibility/Adaptabilty - Precision - Continous learning - Auto-organization - Proactivity - Problem Solving - Team Working

Techniques

Animal Models: Mouse (BALB/c and NSG strains); mouse strains maintenance; tumor cell injection in mammary fat-pad; tumor growth evaluation; tumor and organs explant; blood sample collection; PCR genotyping; sacrifice of animals; PDX management; wide ex-vivo analysis of murine or PDX samples Molecular Biology: PCR; RT-PCR (retrotranscription); qRT-PCR (real-time); western blotting; protein, DNA and RNA purification from biological samples (cell lines, tumorsphere, organoids, primary tumors and organs); immunoprecipi­tation; transfection (Lipofectamine 2000 and PolyJet); ELISA assay; MTT assay; soft-agar assay; immunofluorescence on 2D cells, tumor­spheres and organoids; FACS sample preparation and analysis, cell cycle analysis, apoptosis analysis, drug dose-response experiments Cell cultures: human and murine breast cancer cell cultures (TuBo, SKBR3, 4T1, MDA-MB-231, S68, SUM159, SUM149 cell lines); mammosphere formation assay from cell lines and primary tumors; Platinum A cell culture; organoids cultures from tumor cells and PDX derived cells Microscopy: ApoTome and Time-Lapse microscopes from Zeiss; ViCo microscope; Confocal SP8 microscope from Leica; Confocal LS880 AiryScan from Zeiss lnformatics: Softwares: Microsoft Office (Word, Excel and Power Point); iWork (Pages, Numbers and Keynote); Internet Browsers; lmageJ; lmage Lab; Graph Pad Prism; Mendeley; Adobe Acrobat PRO; Adobe CC Suite; Computer Programming (#C, Perl Language and basic Unix system programming) Operating systems: Windows XP, Windows Vista, Windows 7, Windows 8, Windows 10; Linux (Ubuntu); MacOS (Apple)