Madame Vinothini VIJAYA KUMAR / PhD student

Techniques

• Molecular Biology Techniques: PCR, cloning, plasmid expansion, DNA/RNA quantification, in vitro transcription, transformation, and transfection with LNPs • Protein and Immunoassays: SDS-PAGE, Western blot, ELISA, immunoprecipitation, and immunofluorescence. • Cell Culture and Maintenance: Mammalian cell culture, virus culture, and plaque assay. • RNA-Specific Techniques: RNA quantification, mRNA synthesis, and reverse transcription. • Data Analysis and Bioinformatics: Proficiency in R and Python (machine learning), experience with online bioinformatics tools, and MinION Nanopore sequencing. • Laboratory Techniques: Competent cell preparation, lab-scale culturing, fluorescence microscopy.