Monsieur Guillaume GOTTHARD / PhD


Caractérisation structurale d'enzymes hydrolysant les organophosphorés et rationalisation de leur amélioration en vue d'applications biotechnologiques

Professional contact details

27 Bd Jean Moulin, 13385 Marseille , FRANCE

0609541158

Professional experience

Contract type : Contrat post-doctoral en organisme de recherche (EPST, EPIC, EPA)

Organization or company : ESRF (European Synchrotron Radiation Facility

City : Grenoble

Country : FRANCE

Jobs

Research : NON

Techniques

- diffraction des rayons X, analyse des données, modélisation, analyse des structures 3D, dockings, modélisation d'interactions - cristallisation des protéines (screening de conditions, gouttes suspendues ...) - caractérisation de protéines (filtration sur gel, immunoprécipitations, fluorescence, dichroïsme circulaire, ELISAs de dosage, ELISA d'association, électrophorèses (SDS-PAGE, native, western blot ...) - purification de protéines (chromatographie d'affinité, échangeuses d'ions, filtration sur gel ...) - biologie moléculaire (culture de bactéries, transformation, clonage de vecteurs, PCR, purification sur gel, minipreps, southern blot ...)

PhD

Title : Biologie-Santé: Biochimie structurale

First registration date : 1 October 2009 / 4A

Doctoral school : Sciences de la Vie et de la Santé

Thesis defense date : 29 October 2013

Subject : Caractérisation structurale d'enzymes hydrolysant les organophosphorés et rationalisation de leur amélioration en vue d'applications biotechnologiques

Thesis supervisor : CHABRIERE Eric

Thesis co-supervisor :

Research unit : URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales

Master's degree

Title : Master de Recherche mention Bioinformatique, Biologie Structurale et Génomique

June 2009 - Université Aix-Marseille II

Mention : Bien

Publications

  • Crystallization and preliminary X-ray diffraction analysis of a high affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1
    0
    Gotthard G, Djeghader A, Suh A, Gonzalez D, Scott K, Chabriere E, Elias M

  • Crystallization and preliminary X-ray diffraction analysis of the lactonase VmoLac from Vulcanisaeta moutnovskia
    0
    Gotthard G, Hiblot J, Champion C, Chabriere E, Elias

  • Structural and enzymatic characterization of the phosphotriesterase OPHC2 from Pseudomonas pseudoalcaligenes
    0
    Gotthard G, Hiblot J, Gonzalez D, Robin J, Elias M, Chabriere E

  • Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox
    0
    Gotthard G, Hiblot J, Elias M, Chabriere E

  • Long-range DHPS mutations unexpectedly increase Mycobacterium chimaera susceptibility to sulfonamides
    0
    Gotthard G, Muhamed Ameen S, Drancourt M, Chabriere E

  • Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14.
    0
    Gotthard G, Djeghader A, Suh A, Gonzalez D, Scott K, Elias M, Chabriere E.

  • Crystallization and preliminary X-ray diffraction analysis of the organophosphorus hydrolase OPHC2 from Pseudomonas pseudoalcaligenes.
    0
    Gotthard G, Hiblot J, Gonzalez D, Chabrière E, Elias M.

  • Characterisation of the organophosphate hydrolase catalytic activity of SsoPox.
    0
    Hiblot J, Gotthard G, Chabriere E, Elias M

  • Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus.
    0
    Gotthard G, Hiblot J, Chabriere E, Elias M.

  • The Level of DING proteins is increased in HIV-infected patients: in vitro and in vivo studies
    0
    Ahmed Djeghader, Gerard Aragonès, Nune Darbinian, Mikael Elias, Daniel Gonzalez, Anabel García-Heredia, Raúl Beltrán-Debón, Rafal Kaminski, Guillaume Gotthard; Julien Hiblot, Anna Rull, Olivier Rohr, Christian Schwartz, Carlos Alonso-Villaverde, Jorge Joven, Jordi Camps and Eric Chabriere

  • Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication
    0
    Thomas Cherrier, Mikael Elias, Alicia Jeudy, Guillaume Gotthard, Valentin Le Douce, Houda Hallay, Patrick Masson, Andrea Janossy, Ermanno Candolfi, Olivier Rohr, Eric Chabrière and Christian Schwartz

  • Crystallization and preliminary x-ray diffraction analysis of the hyperthermophilic Sulfolobus islandicus Lactonase
    0
    Gotthard G, Hiblot J, Elias M, Chabriere E

  • Structural insights and ab initio sequencing within the DING proteins family
    0
    Elias M, Liebschner D, Gotthard G , Chabriere E

  • Eukaryotic DING proteins are endogenous: an immunohistological study in mouse tissues
    0
    Collombet JM, Elias M, Gotthard G , Four E, Renault F, Joffre A, Baubichon D, Rochu D, Chabriere E

Languages

Anglais : C1 - Avancé

Allemand : B1 - Intermédiaire