Monsieur Guillaume GOTTHARD / PhD
Caractérisation structurale d'enzymes hydrolysant les organophosphorés et rationalisation de leur amélioration en vue d'applications biotechnologiques
Contact details
Professional contact details
27 Bd Jean Moulin, 13385 Marseille , FRANCE
0609541158
Professional experience
Contract type : Contrat post-doctoral en organisme de recherche (EPST, EPIC, EPA)
Organization or company : ESRF (European Synchrotron Radiation Facility
City : Grenoble
Country : FRANCE
Jobs
Research : NON
Techniques
- diffraction des rayons X, analyse des données, modélisation, analyse des structures 3D, dockings, modélisation d'interactions - cristallisation des protéines (screening de conditions, gouttes suspendues ...) - caractérisation de protéines (filtration sur gel, immunoprécipitations, fluorescence, dichroïsme circulaire, ELISAs de dosage, ELISA d'association, électrophorèses (SDS-PAGE, native, western blot ...) - purification de protéines (chromatographie d'affinité, échangeuses d'ions, filtration sur gel ...) - biologie moléculaire (culture de bactéries, transformation, clonage de vecteurs, PCR, purification sur gel, minipreps, southern blot ...)
PhD
Title : Biologie-Santé: Biochimie structurale
First registration date : 1 October 2009 / 4A
Doctoral school : Sciences de la Vie et de la Santé
Thesis defense date : 29 October 2013
Subject : Caractérisation structurale d'enzymes hydrolysant les organophosphorés et rationalisation de leur amélioration en vue d'applications biotechnologiques
Thesis supervisor : CHABRIERE Eric
Thesis co-supervisor :
Research unit : URMITE - Unité de Recherche sur les Maladies Infectieuses et Tropicales
Master's degree
Title : Master de Recherche mention Bioinformatique, Biologie Structurale et Génomique
June 2009 - Université Aix-Marseille II
Mention : Bien
Publications
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Crystallization and preliminary X-ray diffraction analysis of a high affinity phosphate-binding protein endowed with phosphatase activity from Pseudomonas aeruginosa PAO1
0
Gotthard G, Djeghader A, Suh A, Gonzalez D, Scott K, Chabriere E, Elias M
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Crystallization and preliminary X-ray diffraction analysis of the lactonase VmoLac from Vulcanisaeta moutnovskia
0
Gotthard G, Hiblot J, Champion C, Chabriere E, Elias
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Structural and enzymatic characterization of the phosphotriesterase OPHC2 from Pseudomonas pseudoalcaligenes
0
Gotthard G, Hiblot J, Gonzalez D, Robin J, Elias M, Chabriere E
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Differential active site loop conformations mediate promiscuous activities in the lactonase SsoPox
0
Gotthard G, Hiblot J, Elias M, Chabriere E
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Long-range DHPS mutations unexpectedly increase Mycobacterium chimaera susceptibility to sulfonamides
0
Gotthard G, Muhamed Ameen S, Drancourt M, Chabriere E
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Crystallization and preliminary X-ray diffraction analysis of a DING protein from Pseudomonas aeruginosa PA14.
0
Gotthard G, Djeghader A, Suh A, Gonzalez D, Scott K, Elias M, Chabriere E.
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Crystallization and preliminary X-ray diffraction analysis of the organophosphorus hydrolase OPHC2 from Pseudomonas pseudoalcaligenes.
0
Gotthard G, Hiblot J, Gonzalez D, Chabrière E, Elias M.
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Characterisation of the organophosphate hydrolase catalytic activity of SsoPox.
0
Hiblot J, Gotthard G, Chabriere E, Elias M
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Structural and enzymatic characterization of the lactonase SisLac from Sulfolobus islandicus.
0
Gotthard G, Hiblot J, Chabriere E, Elias M.
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The Level of DING proteins is increased in HIV-infected patients: in vitro and in vivo studies
0
Ahmed Djeghader, Gerard Aragonès, Nune Darbinian, Mikael Elias, Daniel Gonzalez, Anabel García-Heredia, Raúl Beltrán-Debón, Rafal Kaminski, Guillaume Gotthard; Julien Hiblot, Anna Rull, Olivier Rohr, Christian Schwartz, Carlos Alonso-Villaverde, Jorge Joven, Jordi Camps and Eric Chabriere
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Human-Phosphate-Binding-Protein inhibits HIV-1 gene transcription and replication
0
Thomas Cherrier, Mikael Elias, Alicia Jeudy, Guillaume Gotthard, Valentin Le Douce, Houda Hallay, Patrick Masson, Andrea Janossy, Ermanno Candolfi, Olivier Rohr, Eric Chabrière and Christian Schwartz
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Crystallization and preliminary x-ray diffraction analysis of the hyperthermophilic Sulfolobus islandicus Lactonase
0
Gotthard G, Hiblot J, Elias M, Chabriere E
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Structural insights and ab initio sequencing within the DING proteins family
0
Elias M, Liebschner D, Gotthard G , Chabriere E
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Eukaryotic DING proteins are endogenous: an immunohistological study in mouse tissues
0
Collombet JM, Elias M, Gotthard G , Four E, Renault F, Joffre A, Baubichon D, Rochu D, Chabriere E
Languages
Anglais : C1 - Avancé
Allemand : B1 - Intermédiaire