Madame Shobana SANKAR / PhD student

Skills

writing and presentation

Techniques

Molecular Biology and Biochemistry: Immunofluorescence and confocal microscopy; Small RNA sequencing: Ago-APP and library preparation, RT-qPCR; in situ hybridisation; extraction and quantification of DNA, RNA, and protein, western blotting; Plasmid cloning and generating transgenics Bioinformatics and Biostatistics: RNA sequencing analysis: differential expression analysis, target prediction and gene ontology enrichment analysis; Image analysis using ImageJ plugins; Prediction of RNA-protein interaction, SPSS, admixture analysis, GWAS, pathway analysis Others: Maintaining and transfecting HEK293T and SH-SY5Y cell lines, Dissection of the mouse brain cortex, sectioning of fixed post-mortem mouse brain samples, handling and dissection of zebrafish