Monsieur Charbel AL BAYSSARI / PhD

Skills

• Languages - Arabic (mother language) - French (professional level) - English (professional level)

Techniques

General Molecular Biology Technique DNA/RNA Isolation, Plasmid Isolation, Cloning, Transformation, Non-Radioactive labeling and Southern/ Western/ Slot/ Dot blot hybridization, PCR, RT-PCR, Reverse Transcriptase RT-PCR. General Protein Technique Extraction of Proteins, Protein Purification, Sodium Dodecyl Sulfate-polyacrilamide Gel Electrophoresis (SDS-PAGE), Pulsed Field Gel Electrophoresis (PFGE),Chromatography Techniques. Microbiology Technique Isolation, Purification and Maintenance of pure cultures of microorganisms, Culturing in different mediums, Antibacterial assay, Gram staining, MALDI-TOF analysis, Ultraflex assay for detection of carbapenemase production, Transmission Electron Microscopy, Confocal microscopy, Antibiotic susceptibility testing, Multi Locus Sequencing Typing (MLST). Bioinformatics Whole Genome and Resistome analysis, Genome analytical tools (CLC, Artemis, RNammer, t-RNA scan, MAUVE, Infoseq, BRIG), RAST annotation, ARG-ANNOT (Antibiotic resistance gene annotation), Bioinformatics programme (Bioedit, Mega, Expasy), Primer designing, DNA Sequencing and analysis, ChromasPro, Codon code aligner, Blast tools, CLUSTAL W, CLUSTAL X.